Forensic DNA Evidence Matcher & Cold Case Kinship Profiler

Evidence & Physical Conditions

Latent Sample

Case Investigation Name

Sample Age (Years Elapsed) 30 yrs

Initial Shedder Epithelial Cells 1250 cells

Theoretical baseline: ~0.0066 ng genomic DNA per human diploid cell.

Substrate / Surface Material

Annual Degradation Rate (% / yr) 2.1%

PCR Amplification Cycles 32 cycles

Low-Copy Number (LCN) protocol activated at >30 cycles.

Familial DNA Database Search Size 14,500,000

Forensic Quality Standard: Touch DNA degradation follows enzymatic and oxidative purine scission. Amplicons >250 bp drop out first under prolonged exposure.

STR Amplification & Familial Screening

High Loci Recovery

Retained DNA Mass

0.042ng

Sub-nanogram template

Viable STR Loci

18/ 22

4 amplicons degraded

Profile Completeness

81.8%

CODIS core concordant

Familial Match Prob.

0.9994

Second-degree relative

Familial Relationship Likelihood Ratio (LR) Matrix Prior: Random Offender DB Match

Kinship Hypothesis	Identity By Descent (k0, k1, k2)	Shared Loci Expectation	Kinship Posterior Prob