250
0.75
Ancestral Genes 128
Retained Paralog Pairs 78
Collinear Synteny Blocks 9
Dosage Conserved 12.0% Stoichiometric
Subfunctionalized 21.5% Complementary
Neofunctionalized 8.2% Novel Activity
Pseudogenized/Lost 58.3% Fractionation
Scientific Context from Quanta Magazine:
“Many life forms, from spiders and brassicas to barnacles and hagfish, carry evidence of a duplicated genome in the ancient past. This radical genetic mutation is ‘a cheap and easy way to generate a bunch of heritable variation that can be then quickly repurposed for new stuff.’”

When an entire chromosome set doubles simultaneously, gene stoichiometry (relative dosage of macromolecular multi-protein complexes) remains intact. Over millions of years, most duplicate copies suffer pseudogenization through nonfunctionalizing mutations, while surviving paralogs undergo subfunctionalization or evolve new catalytic roles (neofunctionalization).

Synonymous Substitution Rate (Ks) Age Distribution

Decomposing background small-scale gene births from ancient WGD peaks
Peak Ks: 0.64
Interpretation: The red dashed curve isolates the Gaussian burst of paralog divergence created at the WGD event epoch (t=250), contrasting with the exponential decay curve of continuous background gene births and tandem duplications.

Synteny Dot Plot Matrix

Chromosomal collinearity vs. inversions, translocations, and deletions
Conserved Collinear Diagonals
Collinearity tracking: Diagonal streaks demonstrate intact synteny blocks. Off-diagonal points highlight reciprocal translocations, inverted segments, and chromosomal rearrangements accumulated over evolutionary time.

Gene Regulatory Network Stoichiometry Test

Why whole-genome duplication succeeds where single-gene duplication creates deleterious dosage imbalances

Single-Gene Aneuploidy / Tandem Duplication

Duplicating only Subunit A creates unpartnered excess protein, toxic aggregation, and down-regulated multi-protein complexes.

A1
B1
A2
Stoichiometric Ratio: 2A : 1B (Imbalanced - High Fitness Penalty)

Whole-Genome Duplication (Paleopolyploidy)

All subunits of interacting complexes double simultaneously, maintaining 1:1 stoichiometry without precipitating dosage crises.

A1
B1
+
A2
B2
Stoichiometric Ratio: 2A : 2B (1:1 Ratio Conserved - Neutral/Positive Fitness)
0.60
0.22
0.08
0.015
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