hiPSC CRISPRi Perturbation Atlas Simulator

Perturbation Setup

Target Knockdown Gene POU5F1 (OCT4)

CRISPRi Knockdown Intensity 85%

Preset Knockdown Scenarios

Target Insight: POU5F1

POU5F1 encodes OCT4, required for maintaining stem cell pluripotency. Knockdown leads to collapse of core network expression and triggers differentiation pathways.

Pluripotency Core

Epigenetic / Pioneer

Differentiation Markers

Targeted Knockdown

Cell Fate Telemetry

Pluripotency Index 0.18

Network Stability 32.4%

Primary Trajectory Priming

Neural Ectoderm Priming

Self-Renewal / Pluripotency 18%

Neural Ectoderm 68%

Mesoderm 10%

Endoderm 4%

Affected Downstream Genes

Gene	Target Role	Fold Change