Luminescent Super-Resolution Microscopy Simulator

Biological Nanostructure

Chemiluminescent Reaction Kinetics

Photon Budget / Flash (N)850

Reaction Rate Constant (k)0.45

Background Noise (b, photons)12

Structure Spacing / Size (nm)45 nm

Reaction Execution

Data Workbench & Export

Chemiluminescence Mechanism: Chemical excitation triggers transient photon bursts from single probes with zero background phototoxicity or photobleaching, allowing 2D Gaussian fitting to achieve sub-diffraction spatial resolution down to ~12 nm.

Abbe Limit ($d$) 244 nm

Loc. Precision ($\sigma_{loc}$) 14.2 nm

Nyquist Resolution 21.8 nm

Localized Events 420

Separation Status Resolved (2)

Diffraction-Limited Widefield Rayleigh Limit ~244 nm

100 nm

Chemiluminescent SMLM Recon Sub-Diffraction Localization

100 nm