Luminescent Super-Resolution Microscopy Simulator

Chemiluminescence SMLM • Nature Biophysics Model
Biological Nanostructure
Chemiluminescent Reaction Kinetics
Photon Budget / Flash (N)850
Reaction Rate Constant (k)0.45
Background Noise (b, photons)12
Structure Spacing / Size (nm)45 nm
Reaction Execution
Data Workbench & Export
Chemiluminescence Mechanism: Chemical excitation triggers transient photon bursts from single probes with zero background phototoxicity or photobleaching, allowing 2D Gaussian fitting to achieve sub-diffraction spatial resolution down to ~12 nm.
Abbe Limit ($d$) 244 nm
Loc. Precision ($\sigma_{loc}$) 14.2 nm
Nyquist Resolution 21.8 nm
Localized Events 420
Separation Status Resolved (2)
Diffraction-Limited Widefield Rayleigh Limit ~244 nm
100 nm
Chemiluminescent SMLM Recon Sub-Diffraction Localization
100 nm
Live Intensity Cross-Section (Horizontal Cut across Center) ■ Widefield Blur  |  ■ SMLM Reconstruction
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