Mouse Sleep Drive Dual-Cluster Neural Circuit Simulator

Laser: Active (20 Hz)

Target: Cluster A (Somnogenic)

Brain State Classification

Slow-Wave Sleep Induction

Homeostatic flip-flop switch closed

Key Discovery: Cluster A directly suppresses waking nuclei (LC/TMN), while Cluster B stabilizes delta-power slow-wave oscillations.

Electrophysiology Telemetry

Peak Somnogenic Freq

22.4 Hz

Arousal Suppression

78.5%

Sleep Onset Latency

4.8 s

Process S Decay Rate

0.14 /min

Optogenetic & Synaptic Tuning

Laser Pulse Freq20 Hz

Ascending Arousal Drive (Wake Center)0.85

Cluster A GABAergic Weight1.2x

Process S (Adenosine) Build-up0.75

Direct Circuit Interaction

• Click Neurons: Click Cluster A (Cyan), Cluster B (Purple), or Arousal (Red) somas to patch-clamp and inspect single-unit voltage.
• Laser Stimulation: Hold 473nm (ChR2) to excite sleep drive or 589nm (Arch) to suppress somnogenic firing.
• Flip-Flop Model: Observe reciprocal GABAergic inhibition shutting off Locus Coeruleus (LC) noradrenergic firing.