1. Library Configuration
Candidate Design Count
5,250 designs
Experimental validation capacity: over 5,000 parallel designs.
Biophysical Constraints
Target Binding Affinity (Kd)
1.5 nM
Sub-nanomolar to micromolar dissociation constant. Lower is tighter binding.
Thermal Melting Point (Tm)
74.0 °C
Thermodynamic folding stability threshold (>65°C target for cellular assays).
Predicted Solubility Index
0.88
Hydropathy & surface charge balance metric (0.0 to 1.0).
Cell-Free Expression Yield
45.0 mg/L
Microfluidic protein production titer required for automated biophysical validation.
Overall Success Prob.
94.2%
Thermodynamically Favored
Claude Compute Pool
$185,000
From $1.0M Program Pool
Adaptyv Wet-Lab Grant
$50,000
Bio Foundry Synthesis
Validated Yield
4,945
94.2% pass rate
Thermal Denaturation & Binding Kinetics Simulation
Tm: 74.0°C | Kd: 1.5 nM
Multi-Tier High-Throughput Robotic Screening Funnel
Simulating synthesis, expression, circular dichroism folding, and surface plasmon resonance (SPR) binding across the submitted design pool.
Stage 1: Cell-Free Gene Synthesis & Titer (Yield > 15 mg/L)
5,145 / 5,250 (98.0%)
Stage 2: Soluble Monomeric Folding (Solubility > 0.65)
5,042 / 5,250 (96.0%)
Stage 3: Thermal Stability Retention (Tm > 65°C)
5,015 / 5,250 (95.5%)
Stage 4: High-Affinity On-Target Binding (Kd < 5.0 nM)
4,945 / 5,250 (94.2%)
Structured Entry Dossier Manifest
protein_design_dossier_helixsynth.json