CRISPR Base Editing Roadblock Simulator

UW-Madison 19,144-Gene Screen: Resolving Competitive DNA Repair Flux

19,144 Genes Screened

Molecular Target & Deamination R-Loop

52.1%

Editing Efficiency

6.2x

Fold Increase

1.8%

Indel Byproduct

Desired Transition (Productive Edit): 52.1%

BER/MMR Reversion Flux (Roadblock): 46.1%

Top 6 Screened Roadblocks 19,138 Neutral/Other Genes

Toggle targeted disruptions to suppress competing repair pathways:

Simulated Target Locus Trace: ATGCGATCGGTACTA Product Purity: 96.7%