Fleming Penicillin Zone of Inhibition Simulator

1. Experimental Setup

HISTORICAL & CLINICAL PRESETS

INTERACTIVE ACTION TOOL

TARGET BACTERIAL STRAIN

INCUBATION TEMP: 37°C

AGAR NUTRIENT DENSITY: 100%

DRUG DIFFUSION RATE: 1.0x

Fleming 1928 Penicillium Chrysogenum Active Assay

Penicillium mold spore actively secreting penicillin antibiotic into nutrient agar substrate. Observing peptidoglycan cell wall degradation and radial clearance in S. aureus bacterial lawn.

2. Real-Time Telemetry

Max Inhibition Zone

28.5

millimeters (mm)

Bacterial Lawn Density

84.2

% coverage

Incubation Time

18.0

hours elapsed

Resistant Mutations

colonies in zone

🔬 CELL WALL LYSIS INSPECTION 20,000x Magnification

Peptidoglycan Degradation active: Beta-lactam rings bind transpeptidase enzymes (PBPs), preventing cross-linking of peptide chains and triggering osmotic lysis.

Active Bacterial Lawn (Intact Cell Wall)

Zone of Inhibition (Lysis / Cleared)

Penicillin Drug Diffusion Front

Penicillium Mold Mycelium Center

Beta-Lactamase Resistant Mutants