IS110 Bridge Recombination Mechanics Nature Cryo-EM v85

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1. bRNA Docking
2. Dimer Assembly
3. Top Strand Cut
4. 180° Swivel
5. Re-Ligation
Target DNA
Donor DNA
bRNA (TBL/DBL Loops)
RuvC Dimer Core
Stage 1: The dual-loop bridge RNA (bRNA) engages both Target DNA and Donor DNA simultaneously via base pairing in the Target-Binding Loop (TBL) and Donor-Binding Loop (DBL).
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