Diffraction-Limited Widefield (Abbe Limit)
~250 nm Blur
200 nm
Luminescent Super-Resolution (Reconstructed)
18 nm Precision
200 nm
Cross-Sectional Intensity Profile (FWHM Line Transect)
Drag line on super-res canvas to inspect FWHM
Nanoscopy Principle: Chemiluminescent/bioluminescent reactions trigger stochastic, transient single-photon emission bursts without continuous laser illumination. By fitting the 2D Gaussian PSF centroid of isolated bursts frame-by-frame, molecular structures below the 250 nm Abbe diffraction limit are reconstructed down to sub-20 nm.
Acquisition & Specimen
Reaction Kinetics & Optics
Substrate Conc. [S]
2.5 µM
Burst Photon Budget (N)
1500 ph
Background Noise (b)
6 ph/px
Acquisition Speed
10 bursts/step
Cramér-Rao Lower Bound (CRLB)
σ_loc ≈ √[ (s² + a²/12)/N + 8πs⁴b² / (a² N²) ]
Calculated Uncertainty: 11.8 nm
Optical PSF s = 115 nm, Pixel size a = 65 nm