Luminescent Reaction Nanoscopy Simulator

Diffraction-Limited Widefield (Abbe Limit) λ = 530nm, NA = 1.4

Camera integration with optical diffraction blur ($\sim 260\text{ nm}$ Airy disk FWHM) and Poisson shot noise.

Single-Molecule Localization Reconstruction < 20 nm Precision

Centroid localization fitting ($\sigma_{\text{loc}}$) from isolated stochastic chemiluminescent flashes.

Localized Events

1,420

Mean Precision (σ_loc)

14.8 nm

Abbe Limit / Current Res

260 nm / 14.8 nm

Enhancement Factor

17.5 ×

Nyquist Criterion

RESOLVED

Biological Sample Structure:

Substrate Flux [uM]: 12.5 μM

Enzyme Turnover [s⁻¹]: 4.2 s⁻¹

Photon Yield / Burst: 850 γ

Detector Background Noise: 4.5 e⁻

Nanoscopy Principle: Chemiluminescence utilizes catalytic energy rather than toxic laser excitation. By starving the reaction kinetics to stochastic sparse single-molecule bursts, each emitter's point spread function ($\approx 260\text{ nm}$) is isolated in time, enabling Gaussian centroid localization down to $\sim 14.8\text{ nm}$ precision based on Thompson-Mortensen-Webb photon statistics.