Prevotella Oxygen Gradient Lab

Gas Chamber Controls

Species Preset

Chamber Oxygen (O₂): 6.5%

Airway Mucus Depth: 120 µm

Incubation Duration: 24.0 h

Initial Inoculum: 1.0 × 10⁵ CFU

Active Mode: P. melaninogenica

Local Effective O₂ at Niche: 4.1%

● Green = Actively Dividing (Fission) ▲ Amber = Oxidative Stress (ROS Arrest) ✕ Red = Cell Lysis

Microaerophilic Telemetry

Final Biomass (CFU/mL)

8.40 × 10⁶

Doubling Time (Td)

3.8 h

ROS Detox Activity

Moderate / Functional

21% O₂ Half-Life (t½)

6.2 h

Physiological Growth Regime

Active Microaerophilic Proliferation

Paradigm Insight: Unlike strict obligate anaerobes, Prevotella melaninogenica expresses low-level superoxide dismutase & rubrerythrin, allowing efficient cellular doubling in 5–8% O₂ lung tissue micro-environments and transient persistence at atmospheric air.